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2021 Neuropixels Workshop

September 23 - 24, 2021

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Neuropixels probes represent a major advance in neural recording technology. Developed by IMEC in collaboration with the Allen Institute, University College London, and Janelia Research Campus, these probes can simultaneously measure spiking activity from hundreds of neurons distributed across the brain. Neuropixels are currently available to order through Neuropixels.org and are described in our publication in Nature.

At this virtual workshop, attendees will learn how to run acute electrophysiology experiments with Neuropixels probes and how to analyze the data using open-source software.

The workshop will consist of both interactive seminars and virtual lab demos, with the goal of teaching you the skills required to set up Neuropixels experiments in your lab. Emphasis will be placed on multi-probe recordings, which take advantage of the unique capabilities of Neuropixels technology.

We are eager to host a diverse audience, and we encourage trainees, bench scientists, and PIs to apply. Some prior experience with in vivo electrophysiology is assumed, but applicants do not need to have previously used or have placed an order for Neuropixels probes in order to attend.

Workshop Topics

Neuropixels Hardware

  • What tools you’ll need to buy to use Neuropixels in your lab
  • Best practices for handling and maintenance
  • Probe alignment and insertion

Software

  • Data acquisition with Open Ephys and SpikeGLX
  • Spike sorting with Kilosort and phy
  • NWB files and the AllenSDK

Tentative Virtual Workshop Schedule

Thursday, September 23, 2021

Session

Time: Pacific

Zoom meeting opens to participants

7:30am

Participant introductions

8:00-8:45am

Workshop overview and introduction to Neuropixels

8:45-9:15am

Recording across the brain

9:15-9:45am

Break

9:45-10:00am

Overview of Neuropixels pipeline methods

10:00-10:30am

Surgery demonstration

10:30-11:00am

Break

11:00-11:15am

Neuropixels hardware

11:15-11:30am

Calibration/targeting demonstration

11:30-11:45am

Break

11:45am-12:00pm

Probe insertion demonstration

12:00-12:30pm

Q&A

12:30-1:00pm

Friday, September 24, 2021

Session

Time: Pacific

Zoom meeting opens to participants

7:30am

Chronic Neuropixels methods

8:00-8:30am

Open Ephys demonstration

8:30-9:00am

Break

9:00-9:15am

Spike sorting with Kilosort

9:15-9:45am

Phy curation and quality metrics

9:45-10:15am

Break

10:15-10:30am

Large-scale data analysis

10:30-11:00am

AllenSDK and NWB files

11:00-11:30am

Break

11:30am-12:00pm

CCF registration demo

12:00-12:30pm

Q&A

12:30-1:00pm

Details

Start:
September 23 - 7:30 am
End:
September 24 - 1:00 pm
Website:
https://alleninstitute.org/what-we-do/brain-science/events-training/neuropixels-workshop-2021/

Venue

Online

Organizer

Allen Institute for Brain Science