In this webinar, Allen Institute for Immunology researchers will provide an overview of the research conducted by the Allen Institute for Immunology and share how multiplexed multi-modal fixed scRNA-seq assays can overcome challenges found in traditional single cell transcriptomics, including scalability and rare cell-type identification. Further, they will demonstrate how the application of this multiplexed assay to deeply profile cellular populations in human bone marrow can empower unique insight into a continuous developmental trajectory of cells.   

Peter Skene, Marla Glass and Zach Thomson used Gene Expression Flex, which allowed them to:  

• Maximize efficiency and get more out of each run with built-in multiplexing  
• Identify rare cell types without upstream enrichment steps  
• Refine our view of immune cell profiling and differentiation 

Attendees will learn:

• How new highly scalable scRNA-seq technologies enable >1 million cell batches through multiplexing and resolvable doublets 
• How the Allen Institute for Immunology is utilizing automation with liquid handling robots to increase cell recovery and reduce batch to batch variability 
• High level overview of bioinformatics pipeline to jumpstart data analysis from large scale batches 
• How multiplexing enables higher resolution analysis of immune cell developmental trajectories in bone marrow samples 
• Explore aspects of B cell development, from the hematopoietic stem cells to immature B cells, as well as a spectrum of mature B cell populations present in human tissues