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Open for (Cell) Science: Allen Cell Collection
June 11, 2021
Fluorescently-tagged human induced pluripotent stem cell (hiPSC) lines to investigate cell and nuclear organization in differentiation, disease, and regeneration
The Allen Institute for Cell Science is creating a collection of fluorescent protein-tagged hiPS cell lines with the objective to illuminate cell organization and enable observation of cellular behavior from a structural perspective. To date, the Allen Cell Collection consists of >40 edited lines with fluorescent tags labeling all the major cellular organelles, signaling molecules, cardiomyocyte-specific structures, phase transition markers, and transcription factors. We have also released a dCas9 line (dCas9-KRAB) which enables CRISPRi-mediated knockdown of target genes. We have made all of these cell lines and plasmids available to use in the lab, in stem cell cores, or by industry partners. Presented by Kimberly R. Metzler, PhD, Senior Scientific Program Manager.