BBI’s Dr. Danny Miller and the UW’s Nanopore Sequencing Core are providing both targeted and whole genome long read sequencing on the Oxford Nanopore platform to assist other researchers with their projects.
“As new DNA and RNA sequencing technologies are developed that provide a more comprehensive view of genetic variation, it is imperative that researchers and clinicians have access to leading-edge services,” said Miller, a pediatrics and medical genetics resident who will start his own lab at the UW this summer. “Our team is offering both targeted and whole-genome long-read DNA and RNA sequencing and complementary support of human samples and other organisms of interest, to all BBI members.”
That team is utilizing, among other machines, a Nanopore PromethION, Oxford Nanopore’s highest-throughput sequencing device, a scalable instrument that runs multiple flow cells independently and can sequence a human to ~40x coverage using a single flow cell. It is able to generate high quality datasets with average read lengths between 10kb and 60kb, suitable for many long-read applications, including identification of single nucleotide variants and indels, structural variant discovery, and methylation analysis.
